Part:BBa_K3453025:Design
DmMatK Trigger 2.2
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 40
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 40
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 40
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 40
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 40
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
designed using our internal toehold switch pipeline which calls NUPACK [3] and RBS Calculator for switch prediction and ranking [4]. For full details, visit the 'Model' page of our wiki.
Source
DNA synthesis
References
[1] Hassold S, Lowry PP 2nd, Bauert MR, Razafintsalama A, Ramamonjisoa L, Widmer A. DNA barcoding of Malagasy rosewoods: towards a molecular identification of CITES-listed Dalbergia species. PLoS One (2016) 11, e0157881.
[2] Green AA, Silver PA, Collins JJ, Yin P. Toehold switches: de-novo-designed regulators of gene expression. Cell (2014) 159, 925-939.
[3] Zadeh JN, Steenberg CD, Bois JS, Wolfe BR, Pierce MB, Khan AR, Dirks RM, Pierce NA. NUPACK: Analysis and design of nucleic acid systems. Journal of Computational Chemistry (2011) 32, 170–173.
[4] Salis HM. The ribosome binding site calculator. Methods in Enzymology (2011) 498, 19–42.